Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

We have developed and tested a novel ELISA‐like approach for bacterial detection based upon selective adhesion of targeted bacteria to microwells with prefabricated bacterial bioimprints. Bioimprints were produced from three bacterial species; Escherichia coli (Gram‐negative), Rhodococcus rhodochrous (Gram‐positive) and Sarcina aurantiaca (Gram‐negative), by using molding with curable silicone from dense layers of bacterial cells deposited on a...