An in vitro 3D diabetic human skin model from diabetic primary cells.

Published on Dec 17, 2020in Biomedical Materials3.174
· DOI :10.1088/1748-605X/ABC1B1
Candan Yilmaz Ozdogan1
Estimated H-index: 1
(Kocaeli University),
Halime Kenar (Acıbadem University)+ 3 AuthorsSahin Alagoz4
Estimated H-index: 4
(Kocaeli University)
#1Cemile Kilic Bektas (METU: Middle East Technical University)H-Index: 4
#2Vasif HasirciH-Index: 52
Tissue engineering aims to replace missing or damaged tissues and restore their functions. Three-dimensional (3D) printing has been gaining more attention because it enables the researchers to design and produce cell loaded constructs with predetermined shapes, sizes, and interior architecture. In the present study, a 3D bioprinted corneal stroma equivalent was designed to substitute for the native tissue. Reproducible outer and inner organization of the stroma was obtained by optimizing printin...
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Biofabrication with various hydrogel systems allows production of tissue or organ construct in vitro to address various challenges in healthcare and medicine. In particular, photocrosslinkable hydrogels have great advantages such as excellent spatial and temporal selectivity and low processing cost and energy requirement. However, inefficient polymerization kinetics of commercialized photoinitiators upon exposure to UV-A or visible light increase processing time, often compromising cell viabilit...
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Abstract Collagen peptides are widely used in food and medicine industries. Given the significant role of collagen in bone structure, collagen peptides are considered promising for osteoporosis prevention and treatment. To elucidate their effect on osteoporosis, we extracted porcine collagen, digested it into peptides, and investigated their biological function using osteoblast MC3T3-E1 cells. The collagen structure was relatively stable and we prepared peptides with different molecular weights ...
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Purpose: Non-healing or slow healing chronic wounds are among serious complications of diabetes that eventually result in amputation of limbs and increased morbidities and mortalities. Chronic diabetic wounds show reduced blood vessel formation (lack of angiogenesis), inadequate cell proliferation and poor cell migration near wounds. In this paper, we report the development of a hydrogel-based novel wound dressing material loaded with reduced graphene oxide (rGO) to promote cell proliferation, c...
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#1Marline Kirsch (Leibniz University of Hanover)H-Index: 2
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Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic research to better understand intercellular and cell-matrix interactions. Moreover, 3D cell culture plays an increasingly important role in the in vitro testing of bioactive substances and tissue engineering. Gelatin-methacryloyl (GelMA) hydrogels of different degrees of functi...
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Recently, astaxanthin, a red lipophilic pigment belonging to the xanthophyllic family of carotenoids, has shown the feasibility of its uses in tissue engineering and regenerative medicine, due to its excellent antioxidant activities and its abilities to enhance the self-renewal potency of stem cells. In this study, we demonstrate the influence of astaxanthin on the proliferation of adipose-derived mesenchymal stem cells in tissue-engineered constructs. The tissue engineered scaffolds were fabric...
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#1Wenbing Wan (NCU: Nanchang University)H-Index: 2
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Diabetic wounds are characterized by sustained chronic inflammation, and decreased granulation tissue formation and vascularization, and it is a great challenge for researchers to promote chronic wound healing. In this study, we fabricate a bilayer skin substrate composed of a top layer made of silver-loaded gelatine cryogel and a bottom layer made of a platelet-derived growth factor-BB (PDGF-BB) loaded 3D printed gelatine scaffold for diabetic wound healing. In vitro, the concentration of loade...
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Abstract Human skin equivalents (HSEs) are three dimensional models resembling native human skin (NHS) in many aspects. Despite the manifold similarities to NHS, a restriction in its applications is the altered in vitro lipid barrier formation, which compromises the barrier functionality. This could be induced by suboptimal cell culturing conditions, which amongst others is the diminished activation of the vitamin D receptor (VDR) signalling pathway. The active metabolite of this signalling path...
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Abstract Current commercially available human skin equivalents (HSEs) are used for relatively short term studies (∼1 week) due in part to the time-dependent contraction of the collagen gel-based matrix and the limited cell types and skin tissue components utilized. In contrast, here we describe a new matrix consisting of a silk-collagen composite system that provides long term, stable cultivation with reduced contraction and degradation over time. This matrix supports full thickness skin equival...
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#1Halime Kenar (Kocaeli University)H-Index: 12
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Abstract Success of 3D tissue substitutes in clinical applications depends on the presence of vascular networks in their structure. Accordingly, research in tissue engineering is focused on the stimulation of angiogenesis or generation of a vascular network in the scaffolds prior to implantation. A novel, xeno-free, collagen/hyaluronic acid-based poly( l -lactide-co-e-caprolactone) (PLC/COL/HA) (20/9.5/0.5 w/w/w) microfibrous scaffold was produced by electrospinning. Collagen types I and III, an...
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