High‐throughput evolutionary optimization of the induction medium towards recombinant protein production in BY‐2 tobacco

Bright yellow (BY‐2) tobacco cells combined with the XVE chemically inducible system are one of the most promising plant‐based platforms for recombinant protein production. This offers a range of benefits, including the separation of the cell growth and heterologous gene expression, lack of risk of infecting the end product with prions and human viruses or appropriate protein glycosylation and folding. However, low protein productivity remains a...