Precise quantification of the antibacterial activity of chitosan by NB medium neutralizer

In the present research, nutrient broth (NB) medium was identified to be able to neutralize the antibacterial activity of chitosan and its derivatives. Therefore, an improved test method independent of NB medium was proposed to precisely quantify the antibacterial effectiveness and efficiency of chitosan. The minimum bactericidal concentration (MBC) of chitosan was 60 μg mL−1 against S. aureus and E. coli, and 0.01 % (w/v) chitosan could kill...