Original paper
Molecular barcoding of native RNAs using nanopore sequencing and deep learning
Abstract
Nanopore sequencing enables direct measurement of RNA molecules without conversion to cDNA, thus opening the gates to a new era for RNA biology. However, the lack of molecular barcoding of direct RNA nanopore sequencing data sets severely affects the applicability of this technology to biological samples, where RNA availability is often limited. Here, we provide the first experimental protocol and associated algorithm to barcode and demultiplex...
Paper Details
Title
Molecular barcoding of native RNAs using nanopore sequencing and deep learning
Published Date
Sep 1, 2020
Journal
Volume
30
Issue
9
Pages
1345 - 1353