Gene

Metabolite

Murashige and Skoog medium

Cytokinin

Biology

Operating system

Biochemistry

Computer science

Botany

Explant culture

Genetics

Auxin

Micropropagation

In vitro

Shoot

Horticulture

Kinetin

Metabolite profiling

Profiling (computer programming)

Acclimatization

Industrial Crops and Products

The influence of meta-Topolin (mT) on micropropagation of Salvia sclarea L. (clary sage) was compared with N6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ). mT was superior over all other cytokinin for not only shoot regeneration but also rooting. The highest shoot number (4.9 shoots/explants) was noted on nodal segments cultured on Murashige and Skoog (MS) medium including 2.0 mg/L mT and 0.2 mg/L indole-3-acetic acid (IAA). The regenerated shoots derived from mT including medium were rooted successfully on MS medium having 1.0 mg/L 1-naphthaleneacetic acid (NAA). Plantlets were successfully acclimatised (100%) and exhibited vigorous development in the greenhouse. The genetic stability of the propagated plants was assessed by start codon targeted polymorphism (SCoT) primers. A total variability of 3.13% was noted in the regenerants that revealed a high rated of genetic stability among the micropropagated plants. In addition, the secondary metabolite profiling of micropropagated plants was evaluated with gas chromatography-mass spectrometry (GC–MS). Although the hexane extracts of plants were dominated by n-alkanes, cytokinins added in the culture media altered their secondary metabolite contents. The protocol described here is a rapid and reliable micropropagation protocol for large-scale production of clonally stable clary sage plants for commercial purposes.

In vitro propagation of Salvia sclarea L. by meta-Topolin, and assessment of genetic stability and secondary metabolite profiling of micropropagated plants