Molecular Mechanisms of CRISPR-Cas Immunity in Bacteria.

Published on Nov 23, 2020in Annual Review of Genetics11.146
· DOI :10.1146/ANNUREV-GENET-022120-112523
Philip M. Nussenzweig1
Estimated H-index: 1
(Kettering University),
Philip M Nussenzweig4
Estimated H-index: 4
(Kettering University),
Luciano A. Marraffini45
Estimated H-index: 45
(Rockefeller University)
Prokaryotes have developed numerous defense strategies to combat the constant threat posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced short palindromic rep...
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For Type I CRISPR-Cas systems, a mode of CRISPR adaptation named priming has been described. Priming allows specific and highly efficient acquisition of new spacers from DNA recognized (primed) by the Cascade-crRNA (CRISPR RNA) effector complex. Recognition of the priming protospacer by Cascade-crRNA serves as a signal for engaging the Cas3 nuclease-helicase required for both interference and primed adaptation, suggesting the existence of a primed adaptation complex (PAC) containing the Cas1-Cas...
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It’s an evolution of its own kind that a technology changed the interface of biology in such a short expanse of time. Merely a decade ago, scientists reported that the CRISPR-Cas (clustered regularly interspaced short Palindromic repeats-CRISPR associated) system is the part of bacteria and archea’s adaptive immune system which helps in withstanding the attack against invading viruses by acquiring genetic records of invaders to facilitate robust interference upon reinfection. In this Review, we ...
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