Comprehensive off‐line CCC × LC‐DAD‐MS separation of Cyclopia pubescens Eckl. & Zeyh. phenolic compounds and structural elucidation of isolated compounds
Abstract
Introduction The minor phenolic constituents of Cyclopia pubescens Eckl. & Zeyh. are unknown and one dimensional (1D) liquid chromatography (LC) is unable to provide sufficient separation. Methodology A two‐dimensional (2D) LC method incorporating normal‐phasehigh performance countercurrent chromatography (NP‐HPCCC) in the first dimension ( 1 D) and reversed‐phase ultra‐high‐performance liquid chromatography (RP‐UHPLC) as the second...
Paper Details
Title
Comprehensive off‐line CCC × LC‐DAD‐MS separation of Cyclopia pubescens Eckl. & Zeyh. phenolic compounds and structural elucidation of isolated compounds
Published Date
Aug 17, 2020
Journal
Volume
32
Issue
3
Pages
347 - 361
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