Imaging spectroscopy technique was introduced in the cultural heritage field in the 1990s, when a multi-spectral imaging system based on a Vidicon camera was used to identify and map pigments in paintings. Since then, with continuous improvements in imaging technology, the quality of spectroscopic information in the acquired imaging data has greatly increased. Moreover, with the progressive transition from multispectral to hyperspectral imaging techniques, numerous new applicative perspectives have become possible, ranging from non-invasive monitoring to high-quality documentation, such as mapping and characterization of polychrome and multi-material surfaces of cultural properties. This article provides a brief overview of recent developments in the rapidly evolving applications of hyperspectral imaging in this field. The fundamentals of the various strategies, that have been developed for applying this technique to different types of artworks are discussed, together with some examples of recent applications.