Genotypic distribution of Staphylococcus aureus colonizing children and adolescents in daycare centers, an outpatient clinic, and hospitals in a major Brazilian urban setting.

Published on Jul 1, 2020in Diagnostic Microbiology and Infectious Disease2.499
· DOI :10.1016/J.DIAGMICROBIO.2020.115058
Egidio Domingos André Neto1
Estimated H-index: 1
(Federal Fluminense University),
Jaclyn Guerrero1
Estimated H-index: 1
(University of California, Berkeley)
+ 5 AuthorsFábio Aguiar-Alves8
Estimated H-index: 8
(Federal Fluminense University)
Sources
Abstract
Abstract Staphylococcus aureus nasal colonization is a major risk factor for infection. Studies have suggested an epidemiologic shift in the methicillin-resistant S. aureus (MRSA) strains that circulate in Brazil. We conducted cross-sectional studies of MRSA carriage among 1) children and adolescents in community daycare centers, 2) an outpatient clinic, and 3) hospitals in a large Brazilian metropolitan setting. There were 1.500 study subjects, 500 from each locale: 768 (51.2%) carried S. aureus whereas 150 (10%) of these were colonized with MRSA. The most common lineages were the Southwest Pacific (SWP) and the Pediatric clones in all three groups. Roughly 50% of SWP carried Panton-Valentine leukocidin (PVL) (p
📖 Papers frequently viewed together
33 Citations
7 Citations
3 Citations
References33
Newest
#1Fernanda Sampaio Cavalcante (UFRJ: Federal University of Rio de Janeiro)H-Index: 10
#2Marcos Vinicius Pinheiro (UFRJ: Federal University of Rio de Janeiro)H-Index: 1
Last. Kátia Regina Netto dos Santos (UFRJ: Federal University of Rio de Janeiro)H-Index: 23
view all 7 authors...
Background Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are associated with greater mortality and morbidity; however, risk factors for community-acquired infections caused by MRSA have not been established. Therefore, community patients who are admitted to hospitals without the necessary contact precautions and are infected with community-acquired lineages eventually cause these lineages to spread to these settings. The aim of this study was to detect community-acquire...
7 CitationsSource
#1Suzi P. de Carvalho (UFBA: Federal University of Bahia)H-Index: 1
#1Suzi P. de Carvalho (UFBA: Federal University of Bahia)H-Index: 1
Last. Lucas Miranda Marques (USP: University of São Paulo)H-Index: 14
view all 10 authors...
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitoria da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed....
12 CitationsSource
#1Maria Teresa C. Vieira (University of California, Berkeley)H-Index: 1
#2Mariel MarlowH-Index: 12
view all 10 authors...
Background:Methicillin-resistant Staphylococcus aureus (MRSA) colonization has been linked to HIV-related sexual and social behaviors. MRSA risk factors may be different for HIV-infected children, adolescents and young adults. We investigated the association of MRSA colonization, persistent coloniza
9 CitationsSource
#1Chuanling ZhangH-Index: 5
#2Liang GuoH-Index: 1
Last. Stijn van der Veen (ZJU: Zhejiang University)H-Index: 11
view all 8 authors...
The Panton-Valentine leukocidin (PVL) genes of methicillin-resistant Staphylococcus aureus (MRSA) have previously been associated with severe infections. Here, the impact of the PVL genes on severity of disease and clinical outcome of patients with hospital-acquired pneumonia (HAP) or ventilator-associated pneumonia (VAP) due to MRSA was investigated in a single center observational study in a hospital in China. HAP due to MRSA was diagnosed in 100 patients and 13 of the patients were PVL positi...
10 CitationsSource
#1Fernanda Sampaio Cavalcante (UFRJ: Federal University of Rio de Janeiro)H-Index: 10
#2Eliane de Dios Abad (UFRJ: Federal University of Rio de Janeiro)H-Index: 5
Last. K. R. N. dos Santos (UFRJ: Federal University of Rio de Janeiro)H-Index: 6
view all 7 authors...
Staphylococcus aureus is highly prevalent among patients with atopic dermatitis (AD), and this pathogen may trigger and aggravate AD lesions. The aim of this study was to determine the prevalence of S. aureus in the nares of pediatric subjects and verify the phenotypic and molecular characteristics of the isolates in pediatric patients with AD. Isolates were tested for antimicrobial susceptibility, SCCmectyping, and Panton-Valentine Leukocidin (PVL) genes. Lineages were determined by pulsed-fiel...
18 CitationsSource
#1Eneida Dias Vianna Braga (Federal Fluminense University)H-Index: 1
#2Fábio Aguiar-Alves (Federal Fluminense University)H-Index: 8
Last. Claudete Aparecida Araújo Cardoso (Federal Fluminense University)H-Index: 12
view all 12 authors...
Background In the past decade methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent in community settings. Attending a daycare center (DCC) is a known risk factor for colonization with MRSA. Brazil operates free, public DCCs for low-income families, some of which are located in census tracts defined by the Brazilian Census Bureau as informal settlements (aglomerados subnormais, AGSN). Physical and demographic characteristics of AGSNs suggest that S. aureus coloniza...
22 CitationsSource
#1Fabiana Venegas Pires (UNESP: Sao Paulo State University)H-Index: 1
#2Maria de Lourdes Ribeiro de Souza da Cunha (UNESP: Sao Paulo State University)H-Index: 19
Last. Carlos Magno Castelo Branco Fortaleza (UNESP: Sao Paulo State University)H-Index: 17
view all 6 authors...
Recent increases in the incidence and severity of staphylococcal infections renewed interest in studies that assess the burden of asymptomatic carriage of Staphylococcus aureus in the community setting. We conducted a population-based survey in the city of Botucatu, Brazil (122,000 inhabitants), in order to identify the prevalence of nasal carriage of Staphylococcus aureus (including methicillin-resistant strains). Nasal swabs were obtained from 686 persons over one year of age. Resistance to me...
46 CitationsSource
#1Fernanda Sampaio Cavalcante (UFRJ: Federal University of Rio de Janeiro)H-Index: 10
#2Ricardo Pinto Schuenck (UFES: Universidade Federal do Espírito Santo)H-Index: 7
Last. K. R. N. dos Santos (UFRJ: Federal University of Rio de Janeiro)H-Index: 6
view all 6 authors...
Summary This study aimed to characterize meticillin-resistant Staphylococcus aureus (MRSA) lineages circulating in a Brazilian teaching hospital. MRSA isolates from nasal swabs were evaluated to assess antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCC mec ), Panton–Valentine leucocidin status, pulsed-field gel electrophoresis profile and multi-locus sequence type (MLST) analysis. Eighty-three MRSA isolates were analysed. SCC mec III (43.4%) and IV (49.4%) were predominant...
11 CitationsSource
#1Francesco Gesualdo (Boston Children's Hospital)H-Index: 20
#2Dafne Bongiorno (University of Catania)H-Index: 16
Last. Alberto E. TozziH-Index: 32
view all 7 authors...
Background: We report a meta-analysis of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization prevalence in children and a review of the risk factors as well as molecular genetic characterization. Methods: All relevant studies reporting prevalence data on MRSA nasal colonization in children published between January 2000 and August 2010 were retrieved from the MEDLINE database and analyzed. Results: After screening 544 studies, 50 studies were included. We obtained an estimate o...
18 CitationsSource
#1José R. Mediavilla (UMDNJ: University of Medicine and Dentistry of New Jersey)H-Index: 25
#2Liang Chen (UMDNJ: University of Medicine and Dentistry of New Jersey)H-Index: 55
Last. Barry N. Kreiswirth (UMDNJ: University of Medicine and Dentistry of New Jersey)H-Index: 114
view all 4 authors...
During the 1990s, various reports of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections appeared in the literature, caused by novel strains genetically distinct from traditional healthcare-associated MRSA (HA-MRSA). Numerous lineages of CA-MRSA have since emerged on every continent, several of which have spread internationally, most notably USA300. CA-MRSA strains are increasingly implicated in nosocomial infections, and may eventually displace HA-MRSA strains ...
255 CitationsSource
Cited By1
Newest
#1Thamiris Santana Machado (Federal Fluminense University)H-Index: 2
#2Felipe Ramos Pinheiro (Federal Fluminense University)H-Index: 1
Last. Fábio Aguiar-Alves (Federal Fluminense University)H-Index: 8
view all 10 authors...
Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niteroi-Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal c...
2 CitationsSource