A GoldenBraid cloning system for synthetic biology in social amoebae
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain...
Paper Details
Title
A GoldenBraid cloning system for synthetic biology in social amoebae
Published Date
Mar 30, 2020
Journal
Volume
48
Issue
8
Pages
4139 - 4146
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