<h2>Abstract</h2><h3>Statement of problem</h3> Intraoral scanners (IOSs) have some inherent distortions caused by optical and/or software imperfections. However, how other factors such as operator experience, scan time, scanner type, and scan size influence scan accuracy is not clear. <h3>Purpose</h3> The purpose of this in vitro study was to evaluate the trueness and precision of scans performed by 3 professionals with different levels of experience by using 2 IOSs. <h3>Material and methods</h3> Three operators with low, medium, and high levels of experience scanned a master model 10 times by using 2 IOSs (CEREC Omnicam; Dentsply Sirona and TRIOS 3; 3Shape), resulting in 10 standard tessellation language files for each group (N=60). Each standard tessellation language file was divided into 2 areas (prepared teeth and complete arch). Precision was evaluated by comparing the 10 scans from each examiner for each system. Trueness was evaluated by comparing each scan file with a reference scan obtained from a laboratory scanner (D2000; 3Shape). A 3D analysis software program (Geomagic Control; 3D Systems) was used to perform all the comparisons and superimpositions. The 3-way ANOVA test followed by the Tukey HSD test were used to assess precision and trueness. The 2-way ANOVA followed by the Tukey HSD test was used to assess scan time. The Pearson correlation test was performed between scan time and trueness for both scanners. An additional correlation was performed between scan time and number of images, as well as between number of images and trueness for the TRIOS 3. <h3>Results</h3> Statistically significant influences of operator (<i>P</i><.001), scanner (<i>P</i><.001), scan size (<i>P</i><.001), operator and scan size (<i>P</i><.001), and scanner and scan size (<i>P</i><.001) were observed. The TRIOS 3 group reported higher precision than the CEREC Omnicam group for complete-arch scans (<i>P</i><.001), although no difference was observed for scans of the prepared tooth. Medium- (<i>P</i>=.002) and low-experien...