Nocardia

Bacteria

Biology

Microbiology

Nocardiosis

Nocardia Infections

DNA sequencing

Genetics

Medicine

Frontiers in Cellular and Infection Microbiology

In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014-2018 in Huashan hospital and found out that the latter way can not only improved the detection rate of Nocardia spp. but also greatly reduce the turn-around time. In addition, by comparing Nocardiosis and non-Nocardiosis patients both of whose samples had Nocardia spp. detected by NGS, we suggested that Nocardia's specific reads ranking among top 2 might be a satisfactory cut-off value for clinical diagnosis of the disease. Our study introduced the promising value of the NGS method in the rapid diagnosis of nocardiosis.

Rapid Detection of Nocardia by Next-Generation Sequencing