Bioinformatics

Biology

Gene

Cancer

Genome editing

Cas9

T-cell receptor

Immunology

T cell

Computational biology

Multiplex

CRISPR

Cancer research

Genetics

Immune system

Science

CRISPR-Cas9 gene editing provides a powerful tool to enhance the natural ability of human T cells to fight cancer. We report a first-in-human phase 1 clinical trial to test the safety and feasibility of multiplex CRISPR-Cas9 editing to engineer T cells in three patients with refractory cancer. Two genes encoding the endogenous T cell receptor (TCR) chains, TCRα (

CRISPR-engineered T cells in patients with refractory cancer