Polysaccharide

Humoral immunity

Biology

Microbiology

Cytotoxicity

Innate immune system

Chemistry

Mushroom

In vitro

Immunology

Lentinula

Immunity

Edible mushroom

Food science

Biochemistry

Immune system

Journal of Functional Foods

Lentinula edodes (L. edodes) is a type of edible mushroom which is known to beneficially support the immune system. In this study, three polysaccharide fractions (F1, F2 and F3) were isolated from L. edodes water extracts. Their molecular weights distribution (F1 > F2 > F3) and their immunomodulatory properties were investigated. Thymus index, thickness of footpad, proliferation of splenocytes, immunoglobulin (Ig) content and cytotoxicity of natural killer (NK) cells were measured to evaluate the immunomodulatory effects of the polysaccharides. All three polysaccharide fractions protected host from immunosuppression. F1 had significant effects in enhancing cellular immunity only, while F2 and F3 improved cellular immunity, humoral immunity and innate immunity. The immunomodulatory effects became more significant in the order F1 < F2 < F3, inferring that molecular weight was an important factor for bioactivity of the L. edodes polysaccharide fractions. This potential of these mushrooms extracts qualifies them as candidates for immunomodulation in many types of diseases.

Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts