Skin permeability barrier formation by the ichthyosis-causative gene FATP4 through formation of the barrier lipid ω- O -acylceramide

Significance Acylceramide is essential for skin permeability barrier formation. However, its biosynthesis pathway has not yet been elucidated in its entirety. In the present study, we found that Fatp4 disruption substantially decreased the amount of acylceramides in mice, as did FATP4 knockdown in human keratinocytes. In addition, in vitro experiments demonstrated that FATP4 exhibited acyl-CoA synthetase activity toward an ω-hydroxy...