Gene

Function (biology)

Cell biology

Engineering

Biotechnology

Bacteria

Biology

Isolation (microbiology)

Mineralogy

Inclusion (mineral)

Archaeology

Biochemistry

Computer science

Computational biology

Paleontology

Biochemical engineering

Genetics

Bioinformatics

Bacterial protein

Geography

Microbiology

Escherichia coli

Bioprocess

Treasure

Chemistry

Recombinant DNA

Inclusion bodies

Trends in biotechnology

Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving tremendous attention for many diverse applications in the areas of industrial and medical biotechnology. Understanding the structure–function relationship of protein in IBs has recently created new possibilities in developing innovative isolation, solubilization, refolding, and purification processes for high-throughput recovery of bioactive protein from bacterial IBs. This opinion article describes the advantages, disadvantages, and major challenges presently associated with each of the processing steps. Finally, we conclude with the possible solutions for each operational step and the future direction of the basic and translational research to achieve maximum benefit from IB aggregates.

Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins