Evaluation of the effects of keratin on physical, mechanical and biological properties of poly (3-hydroxybutyrate) electrospun scaffold: Potential application in bone tissue engineering
Abstract Proper selection of materials and methods of construction are important factors in scaffolding in tissue engineering. In this research, a novel biomimetic scaffold containing poly (3-hydroxybutyrate) (PHB) and keratin (5-15wt. %) was prepared by the electrospinning method. The results showed that all electrospun scaffolds have 3D structures with high interconnected porosity and hydrophilicity. FTIR, Raman and H-NMR indicated the presence of keratin in electrospun scaffolds and the hydrogen bond between the two polymers. Crystallinity of the scaffolds, which was measured by DSC and verified by XRD, demonstrates that the presence of keratin reduces the crystallinity and improves the thermal behavior of scaffolds. According to the mechanical evaluation, by adding up to 10 wt. % of keratin, tensile strength of scaffolds is increased from 2.29 to 2.92 Mpa, respectively. In vitro degradation of the scaffolds was increased by the presence of keratin. Cell culture of the MG-63 revealed that viability, proliferation and ALP secretion are significantly increased by adding keratin to the scaffolds. The proper properties of PHB-10k electrospun scaffold make it a promising alternative biomaterial for bone tissue engineering applications.
AbstractDesigning scaffolds with appropriate mechanical properties is a challenge in tissue engineering. In this study the poly e-caprolactone (PCL)/gelatin with 1 wt.% of multi-walled carbon nanotubes (MWNTs) fabricated through electrospinning method. The presence of MWNTs led to an increase in the hydrophilicity and tensile strength, while maintaining an appropriate level of porosity percentage. The bioactivity and biodegradation evaluation demonstrated that the scaffolds containing MWNTs pres...
#2Saeed Karbasi(IUMS: Isfahan University of Medical Sciences)H-Index: 21
Last. Laleh Ghasemi(IUT: Isfahan University of Technology)H-Index: 1
view all 4 authors...
ABSTRACTIn this study the poly (ɛ-caprolactone) (PCL)/gelatin nanofibrous scaffolds were fabricated through electrospinning by varying the weight ratio of PCL and gelatin concentrations (90:10, 80:...
Abstract Fibrous scaffolds based on biodegradable piezoelectric poly(3-hydroxybutyrate) (PHB) polymers were fabricated via electrospinning. Hydrothermal deposition of zinc oxide (ZnO) on the surfaces of fibrous PHB scaffolds resulted in a homogeneous ZnO layer that grew conformally on the porous polymeric scaffold. X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results confirmed the formation of a hexagonal wurtzite crystal structure of ZnO on the PHB fibres. XRD patterns, TE...
#2Saeed Karbasi(IUMS: Isfahan University of Medical Sciences)H-Index: 21
Last. Mohammad Rafienia(IUMS: Isfahan University of Medical Sciences)H-Index: 20
view all 4 authors...
Abstract The choice of material types for tissue engineering scaffolds and the design of methods are contributive in yielding the proper result. In this study, 1–5% wt. Alumina nanowires are added to (Polyhydroxybutyrate-Chitosan) PHB-CTS alloy solution, and the scaffolds are prepared by electrospinning method. The fiber diameters, porosity percentages and uniform distribution of Alumina nanowires are assessed by SEM, EDS and TEM. The surface roughness of the fibers is confirmed by FESEM and AFM...
#1Mulugeta Gizaw(UT Tyler: University of Texas at Tyler)H-Index: 2
#2Addison Faglie(UT Tyler: University of Texas at Tyler)H-Index: 3
Last. Shih-Feng Chou(UT Tyler: University of Texas at Tyler)H-Index: 11
view all 5 authors...
Stem cell therapy has emerged as one of the topics in tissue engineering where undifferentiated and multipotent cells are strategically placed/ injected in tissue structure for cell regeneration. Over the years, stem cells have shown promising results in skin repairs for non-healing and/or chronic wounds. The addition of the stem cells around the wound site promotes signaling pathways for growth factors that regulate tissue reconstruction. However, injecting stem cells around the wound site has ...
Abstract Hybrid biodegradable piezoelectric electrospun fibrous scaffolds based on polyhydroxybutyrate (PHB) and polyaniline (PANI, emeraldine salt) were fabricated. Fourier transform infrared spectroscopy and X-ray diffraction confirmed the presence of PANi in the structure of hybrid PHB-based scaffolds. The piezoelectric coefficient d 33 and surface electric potential under cyclic mechanical stress at the frequency of 4 Hz were increased in 4.2 times and 3.5 times, respectively, in the case of...
: Implants of materials that are typically considered inert have been shown to cause early inflammatory complications. In addition, implant wear products may also cause overproduction of proinflammatory cytokines in the long run. Among the cytokines is tumor necrosis factor alpha (TNFα), which not only participates in the inflammatory response but also in the degradation of the bone. Therefore, a lack of production of TNFα by the cells of the immune system in contact with a candidate material fo...
#1Dan Kai(Agency for Science, Technology and Research)H-Index: 32
#2Hui Moon Chong(NUS: National University of Singapore)H-Index: 1
Last. Xian Jun Loh(Agency for Science, Technology and Research)H-Index: 83
view all 9 authors...
Abstract Poly(3-hydroxybutyrate) (PHB) is an attractive biopolymer potential for various applications, but its brittle nature is a big handicap. In this study, we proposed lignin copolymers as mechanical reinforcement agents for PHB. A series of lignin copolymers (random and block) were synthesized via the solvent free ring-opening polymerization (ROP) of β-butyrolactone and/or e-caprolactone onto lignin core. The lignin copolymers were characterized by nuclear magnetic resonance (NMR), gel perm...
#1Moein Zarei(IAU: Islamic Azad University)H-Index: 2
#1Moein Zarei(IAU: Islamic Azad University)H-Index: 4
Last. Saeed Karbasi(IUMS: Isfahan University of Medical Sciences)H-Index: 21
view all 2 authors...
In this study, regarding the importance of optimal design and unique role of a scaffold in tissue regeneration and repair, poly(hydroxybutyrate) (PHB)/multiwalled carbon nanotubes (CNTs) nanocomposite scaffolds with different samples concentrations of CNTs (0, 0.5, 0.75, 1.0, and 1.25% w/v) was prepared by electrospinning technique. Morphological evaluation of scaffolds by using scanning electron microscopy showed the pore volume in all the scaffolds was over 80% and the addition of CNTs increas...
Abstract Sterilization, cytotoxicity and cell viability are essential properties defining a material for medical applications and these characteristics were investigated for poly(β-hydroxybutyrate) (PHB) of 230 kDa obtained by bacterial synthesis from a mutant strain of Azotobacter vinelandii . Cell viability was investigated for two types of PHB scaffolds, solution cast films and non-woven electrospun fibrous membranes, and the efficiency was compared against a culture dish. The biosynthesized ...
Synthetic plastics generate major problems in landfills after their consumption for occupying high volumes and difficult the decomposition of other organic materials. In addition, they are also responsible for impacts on the marine ecosystem. Because of this, the need for cleaner products and process is increasing. Among a variety of existing bioplastics, those derived from cleaner fermentation processes such as polyhydroxyalkanoates (PHA) and polylactic acid (PLA) are promising sustainable alte...
Large-sized or deep skin wounds require skin substitutes for proper healing without scar formation. Therefore, multi-layered skin substitutes that mimic the genuine skin anatomy of multiple layers have attracted attention as suitable skin substitutes. In this study, a novel skin substitute was developed by combining the multi-layer skin tissue reconstruction method with the combination of a human-derived keratinic extract-loaded nano- and micro-fiber using electrospinning and a support structure...
Abstract null null In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated. PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rot...
Biopolymers have been extensively studied as biomaterials for clinical applications due to the specific advantages, such as ample natural abundance, outstanding biocompatibility and biodegradability, and non-inflammatory and enormous functional groups. Protein-based biomaterials have been used in many biotechnology and biomedicine applications. This article briefly introduces the structure and extraction of filament proteins, keratin, and then focuses on the recent researches on the application ...
Abstract Here, we fabricated the electrospun scaffolds (so called PCL/Kr and PCL/Kr/CNT) based on poly(e-caprolactone) (PCL), keratin (Kr), and carboxylated multiwalled carbon nanotubes (CNTs-COOH). Our goal was to evaluate the effect of CNTs-COOH on the osteogenic differentiation and mechanical and physicochemical properties of the scaffolds. We found that the addition of CNTs-COOH to the PCL/Kr scaffold reduced the average fiber diameter from 123 to 55 nm. As a result, the specific surface are...
Research into the development of sustainable biomaterials is increasing in both interest and global importance due to the increasing demand for materials with decreased environmental impact. This research field utilises natural, renewable resources to develop innovative biomaterials. The development of sustainable biomaterials encompasses the entire material life cycle, from desirable traits, and environmental impact from production through to recycling or disposal. The main objective of this re...
Polyhydroxybutyrate (PHB) scaffolds, produced by electrospinning and coated by plasma polymerization of pyrrole, were used as cell culture substrate for beta cells. PHB is a natural polymer that is biocompatible and bioresorbable. The electrospinning conditions used, produced scaffolds with large surface area and high porosity. The scaffold surface was modified by plasma polymerization of pyrrole doped with iodine (PPyI). The morphology of the scaffolds was characterized by scanning electron mic...
#2Hafez Jafari(ULB: Université libre de Bruxelles)H-Index: 5
Last. Amin Shavandi(ULB: Université libre de Bruxelles)H-Index: 17
view all 7 authors...
Electrospun poly (l-lactide-co-d, l-lactide) (PLDLLA)/poly (vinyl alcohol) (PVA) nanofibers were reinforced by various contents (0-1 wt%) of phospho-calcified cellulose nanowhiskers (PCCNWs) as scaffolds in bone applications. The hydrophilicity and rate of hydrolytic degradation of PLDLLA were improved by introducing 10 wt% of PVA. PCCNWs with inherent hydrophilic properties, high aspect ratio, and large elastic modulus enhanced the hydrophilicity, accelerated the rate of degradation, and improv...