Internal medicine

Protein kinase B

Cell culture

Biology

Gene

Cancer

microRNA

Kinase

Chemistry

Esophageal cancer

Transfection

MAPK/ERK pathway

Cell growth

Signal transduction

Cell biology

Protein kinase A

Cancer research

Genetics

Biochemistry

Medicine

PI3K/AKT/mTOR pathway

The Kaohsiung Journal of Medical Sciences

Esophageal cancer is considered as one of the leading malignancies. MicroRNA‐574‐3p (miR‐574‐3p) was used as a postoperative prognostic indicator in patients with esophageal squamous cell carcinoma. However, the underlying mechanism miR‐574‐3p involvement in esophageal cancer remains unclear. In this study, the expression of miR‐574‐3p was reduced in esophageal cancer tissues and cells. In vitro, miR‐574‐3p mimics and inhibitor were transfected into esophageal cancer cells (TE‐1 and TE‐8 cells) to up‐ or downregulating of miR‐574‐3p. miR‐574‐3p inhibited proliferation, migration and invasion, and promoted apoptosis in esophageal cancer cells. In addition, miR‐574‐3p was confirmed to target family with sequence similarity 3 member C (FAM3C) and mitogen‐activated protein kinase 1 (MAPK1). miR‐574‐3p suppressed phosphoinositide 3‐kinase (PI3K)/protein kinase B (AKT) and rapidly accelerated fibrosarcoma (Raf)/mitogen‐activated protein kinase (MEK)/extracellular signal‐regulated kinase (ERK) signaling via regulating FAM3C and MAPK1. In vivo, overexpression of miR‐574‐3p suppressed tumor growth in mice. Our findings indicated that miR‐574‐3p repressed proliferation and invasion of esophageal cancer via regulation of FAM3C and MAPK1, which provides a new biomarker for esophageal cancer treatment.

miR‐574‐3p inhibits proliferation and invasion in esophageal cancer by targeting FAM3C and MAPK1