Capillary electrophoresis‐integrated immobilized enzyme microreactor with graphene oxide as support: Immobilization of negatively charged L ‐lactate dehydrogenase via hydrophobic interactions

We report the first application of hydrophobic interaction between graphene oxide (GO) and negatively charged enzymes to fabricate CE‐integrated immobilized enzyme microreactors (IMERs) by a simple and reliable immobilization procedure based on layer by layer assembly. L ‐lactate dehydrogenase ( L ‐LDH), which is negatively charged during the enzymatic reaction, is selected as the model enzyme. Various spectroscopic techniques, including SEM,...