Differential cell-type-expression of CYFIP1 and CYFIP2 in the adult mouse hippocampus

Published on Nov 24, 2019in Animal Cells and Systems0.907
· DOI :10.1080/19768354.2019.1696406
Yinhua Zhang9
Estimated H-index: 9
(KU: Korea University),
Hyae Rim Kang3
Estimated H-index: 3
(KU: Korea University),
Kihoon Han17
Estimated H-index: 17
(KU: Korea University)
Sources
Abstract
ABSTRACTRecent molecular genetic studies have suggested that two members of the cytoplasmic FMR1-interacting protein (CYFIP) gene family, CYFIP1 and CYFIP2, are causally associated with several bra...
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#1Yinhua Zhang (KU: Korea University)H-Index: 9
#2Yeunkum Lee (KU: Korea University)H-Index: 12
Last. Kihoon Han (KU: Korea University)H-Index: 17
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: The cytoplasmic FMR1-interacting protein family (CYFIP1 and CYFIP2) are evolutionarily conserved proteins originally identified as binding partners of the fragile X mental retardation protein (FMRP), a messenger RNA (mRNA)-binding protein whose loss causes the fragile X syndrome. Moreover, CYFIP is a key component of the heteropentameric WAVE regulatory complex (WRC), a critical regulator of neuronal actin dynamics. Therefore, CYFIP may play key roles in regulating both mRNA translation and ac...
10 CitationsSource
#1Claudia Bagni (UNIL: University of Lausanne)H-Index: 8
#2R. Suzanne Zukin (Albert Einstein College of Medicine)H-Index: 61
Altered synaptic structure and function is a major hallmark of fragile X syndrome (FXS), autism spectrum disorders (ASDs), and other intellectual disabilities (IDs), which are therefore classified as synaptopathies. FXS and ASDs, while clinically and genetically distinct, share significant comorbidity, suggesting that there may be a common molecular and/or cellular basis, presumably at the synapse. In this article, we review brain architecture and synaptic pathways that are dysregulated in FXS a...
87 CitationsSource
CYFIP2, encoding the evolutionary highly conserved cytoplasmic FMRP interacting protein 2, has previously been proposed as a candidate gene for intellectual disability and autism because of its important role linking FMRP-dependent transcription regulation and actin polymerization via the WAVE regulatory complex (WRC). Recently, de novo variants affecting the amino acid p.Arg87 of CYFIP2 were reported in four individuals with epileptic encephalopathy. We here report 12 independent patients harbo...
15 CitationsSource
#1Yinhua Zhang (KU: Korea University)H-Index: 9
#2Hyojin KangH-Index: 23
Last. Kihoon Han (KU: Korea University)H-Index: 17
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Cytoplasmic FMR1-interacting protein 2 (CYFIP2) is a key component of the WAVE regulatory complex (WRC) which regulates actin polymerization and branching in diverse cellular compartments. Recent whole exome sequencing studies identified de novo hotspot variants in CYFIP2 from patients with early-onset epileptic encephalopathy and microcephaly, suggesting that CYFIP2 may have some functions in embryonic brain development. Although perinatal lethality of Cyfip2-null (Cyfip2-/-) mice was reported,...
9 CitationsSource
#1Jing Peng (CSU: Central South University)H-Index: 18
#2Ying Wang (CSU: Central South University)H-Index: 7
Last. Nan Pang (CSU: Central South University)H-Index: 9
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AIMS: West syndrome (WS) is a classic form of early infantile epileptic encephalopathy (EIEE) characterized by tonic spasms with clustering, arrest of psychomotor development, and hypsarrhythmia on electroencephalography. Genetic defects play a critical role in the pathology of WS, and 54 EIEE genes have been identified till date. This study was designed to uncover new candidate genes for West syndrome. METHODS: In this study, we recruited 56 Chinese families with WS of unknown etiology. Whole e...
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#1Arpiar Saunders (Broad Institute)H-Index: 20
#2Evan Z. Macosko (Broad Institute)H-Index: 24
Last. Steven A. McCarroll (Broad Institute)H-Index: 95
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Summary The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features o...
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#1Mitsuko NakashimaH-Index: 40
#2Mitsuhiro KatoH-Index: 55
Last. Naomichi MatsumotoH-Index: 79
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OBJECTIVE: The cytoplasmic fragile X mental retardation 1 interacting proteins 2 (CYFIP2) is a component of the WASP-family verprolin-homologous protein (WAVE) regulatory complex, which is involved in actin dynamics. An obvious association of CYFIP2 variants with human neurological disorders has never been reported. Here, we identified de novo hotspot CYFIP2 variants in neurodevelopmental disorders and explore the possible involvement of the CYFIP2 mutants in the WAVE signaling pathway. METHODS:...
30 CitationsSource
#1Yeunkum Lee (KU: Korea University)H-Index: 12
#2Doyoun KimH-Index: 16
Last. Kihoon HanH-Index: 17
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Actin dynamics is a critical mechanism underlying many cellular processes in neurons. The heteropentameric WAVE-regulatory complex (WRC), consisting of WAVE, CYFIP1/2, Nap, Abi, and HSPC300, is a key regulator of actin dynamics that activates the Arp2/3 complex to initiate actin polymerization and b
12 CitationsSource
#1Leeyup Chung (Duke University)H-Index: 12
#2Xiaoming Wang (Duke University)H-Index: 67
Last. Yong-hui Jiang (Duke University)H-Index: 48
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CYFIP1 maps to the interval between proximal breakpoint 1 (BP1) and breakpoint 2 (BP2) of chromosomal 15q11-q13 deletions that are implicated in the Angelman (AS) and Prader–Willi syndrome (PWS). There is only one breakpoint (BP3) at the distal end of deletion. CYFIP1 is deleted in AS patients with the larger class I deletion (BP1 to BP3) and the neurological presentations in these patients are more severe than that of patients with class II (BP2 to BP3) deletion. The haploinsufficiency of CYFIP...
17 CitationsSource
#1Kihoon Han (Boston Children's Hospital)H-Index: 17
#2Hogmei Chen (Boston Children's Hospital)H-Index: 1
Last. Huda Y. ZoghbiH-Index: 133
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Silencing of fragile X mental retardation 1 (FMR1) gene and loss of fragile X mental retardation protein (FMRP) cause fragile X syndrome (FXS), a genetic disorder characterized by intellectual disability and autistic behaviors. FMRP is an mRNA-binding protein regulating neuronal translation of target mRNAs. Abnormalities in actin-rich dendritic spines are major neuronal features in FXS, but the molecular mechanism and identity of FMRP targets mediating this phenotype remain largely unknown. Cyto...
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Cited By7
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#1Ísis Venturi Biembengut (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 1
#2Isabelle Leticia Zaboroski Silva (FIOCRUZ: Oswaldo Cruz Foundation)
Last. Patrícia Shigunov (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 7
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In humans, the cytoplasmic FMR1 interacting protein (CYFIP) family is composed of CYFIP1 and CYFIP2. Despite their high similarity and shared interaction with many partners, CYFIP1 and CYFIP2 act at different points in cellular processes. CYFIP1 and CYFIP2 have different expression levels in human tissues, and knockout animals die at different time points of development. CYFIP1, similar to CYFIP2, acts in the WAVE regulatory complex (WRC) and plays a role in actin dynamics through the activation...
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#1Anaïs Begemann (UZH: University of Zurich)H-Index: 5
#2Heinrich Sticht (FAU: University of Erlangen-Nuremberg)H-Index: 63
Last. Anita Rauch (UZH: University of Zurich)H-Index: 74
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A few de novo missense variants in the cytoplasmic FMRP-interacting protein 2 (CYFIP2) gene have recently been described as a novel cause of severe intellectual disability, seizures, and hypotonia in 18 individuals, with p.Arg87 substitutions in the majority. We assembled data from 19 newly identified and all 18 previously published individuals with CYFIP2 variants. By structural modeling and investigation of WAVE-regulatory complex (WRC)-mediated actin polymerization in six patient fibroblast l...
4 CitationsSource
The cytoplasmic fragile X mental retardation 1 (FMR1)-interacting protein 2 (CYFIP2) gene is associated with epilepsy, intellectual disability (ID), and developmental delay, suggesting its critical role in proper neuronal development and function. CYFIP2 is involved in regulating cellular actin dynamics and also interacts with RNA-binding proteins. However, the adult brain function of CYFIP2 remains unclear because investigations thus far are limited to Cyfip2 heterozygous (Cyfip2+/- ) mice owin...
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#1Seung-Hyun Lee (SNU: Seoul National University)H-Index: 13
#2Yinhua Zhang (KU: Korea University)H-Index: 9
Last. Kihoon Han (KU: Korea University)H-Index: 17
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OBJECTIVE Genetic variants of the cytoplasmic FMR1-interacting protein 2 (CYFIP2) encoding an actin-regulatory protein are associated with brain disorders, including intellectual disability and epilepsy. However, specific in vivo neuronal defects and potential treatments for CYFIP2-associated brain disorders remain largely unknown. Here, we characterized Cyfip2 heterozygous (Cyfip2+/- ) mice to understand their neurobehavioral phenotypes and underlying pathological mechanisms. Furthermore, we ex...
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#1Sun Kyung Lee (UPR-RP: UPRRP College of Natural Sciences)H-Index: 1
#2Joohong Ahnn (UPR-RP: UPRRP College of Natural Sciences)H-Index: 2
The regulator of calcineurin (RCAN) was first reported as a novel gene called DSCR1, encoded in a region termed the Down syndrome critical region (DSCR) of human chromosome 21. Genome sequence comparisons across species using bioinformatics revealed three members of the RCAN gene family, RCAN1, RCAN2, and RCAN3, present in most jawed vertebrates, with one member observed in most invertebrates and fungi. RCAN is most highly expressed in brain and striated muscles, but expression has been reported...
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#1Yeunkum Lee (KU: Korea University)H-Index: 12
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Last. Kihoon Han (KU: Korea University)H-Index: 17
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Abstract Variants of the cytoplasmic FMR1-interacting protein 2 (CYFIP2) gene are associated with early-onset epileptic encephalopathy, intellectual disability, and developmental delay. However, the current understanding of the molecular functions of CYFIP2 is limited to those related to actin dynamics, and thus, the detailed mechanisms of CYFIP2-associated brain disorders remain largely unknown. Here, we isolated the neonatal forebrain CYFIP2 complex using newly generated Cyfip2-3×Flag knock-in...
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#1Yinhua Zhang (KU: Korea University)H-Index: 9
#2Yeunkum Lee (KU: Korea University)H-Index: 12
Last. Kihoon Han (KU: Korea University)H-Index: 17
view all 3 authors...
: The cytoplasmic FMR1-interacting protein family (CYFIP1 and CYFIP2) are evolutionarily conserved proteins originally identified as binding partners of the fragile X mental retardation protein (FMRP), a messenger RNA (mRNA)-binding protein whose loss causes the fragile X syndrome. Moreover, CYFIP is a key component of the heteropentameric WAVE regulatory complex (WRC), a critical regulator of neuronal actin dynamics. Therefore, CYFIP may play key roles in regulating both mRNA translation and ac...
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