To quantitatively assess the association of problematic smartphone use (PSU) with poor sleep quality, depression, and anxiety, we reviewed current evidence from observational studies and performed a systematic review and meta-analysis. The EBSCO PsycARTICLES, EMBASE, PubMed and Web of Science databases were searched, and 14 studies were included for the systematic review and meta-analysis. We calculated odds risk and 95% confidence intervals (CIs), using random-effect or fixed-effect models. Heterogeneity was assessed using I 2 and chi 2 statistics. Subgroup analyses were conducted based on country, adjusted variables, the methods of statistical analysis, outcome measures and publication year. We found significantly increased risks of poor sleep quality, depression, and anxiety in people with PSU. Our findings highlight the necessity of managing the PSU. However, further studies are required to confirm the results due to the high heterogeneity and methodological limitations.