Preparation and characterizations of three-dimensional porous collagen/graphene oxide/hydroxyapatite nanocomposite scaffolds for bone tissue engineering

Published on Oct 23, 2019
· DOI :10.23954/OSJ.V4I1.2123
Bhahat Lawlley Zimba3
Estimated H-index: 3
(WUT: Wuhan University of Technology),
Jiang Hao2
Estimated H-index: 2
+ 5 AuthorsQingzhi Wu19
Estimated H-index: 19
Studies have reported that the incorporation of graphene oxide (GO) and hydroxyapatite (HA) into biocompatible polymers (such as collagen (Col), chitosan, alginate, etc) results in enhanced structural and mechanical properties respectively. The objective of this study was to prepare and characterize three-dimensional (3D) porous Col/GO/HA nanocomposite scaffolds and to investigate cytocompatibility and osteogenic differentiation potential of rat bone marrow mesenchymal stem cells (rBMSCs) on the as-prepared scaffolds. The SEM images revealed that the scaffolds were porous with the pore diameter inversely proportional to the concentration of HA. XRD results were able to depict the characteristic peaks for HA which shows that HA was incorporated into the scaffolds. The rBMSCs which were cultured on the scaffolds were able to attach and proliferate during the 21 days of the experiment which indicates that the as-prepared scaffolds are cytocompatible. The Alizarin red staining demonstrated the presence of calcium deposits as there were orange-red stains on the samples after culturing the cells using the osteogenic differentiation medium. These results demonstrate the promising potential of the 3D porous Col/GO/HA nanocomposite scaffolds for applications in bone tissue engineering.
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#1Cijun Shuai (Jiangxi University of Science and Technology)H-Index: 42
#2Peng Bo (CSU: Central South University)H-Index: 1
Last. Anjie Min (CSU: Central South University)H-Index: 7
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Abstract Introduction It is urgently needed to develop composite bone scaffold with excellent mechanical properties and bioactivity in bone tissue engineering. Combining graphene oxide (GO) and hydroxyapatite (HAP) for the reinforcement of biopolymer bone scaffold has emerged as a promising strategy. However, the dispersion of GO and HAP remains to be a big challenge. Objectives In this present work, the mechanical properties of GO and the bioactivity of and HAP were combined respectively via in...
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#1Selwa Mokhtar Boularaoui (Khalifa University)H-Index: 3
#3Kamran A. Khan (Khalifa University)H-Index: 17
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