Gene

Biology

Cas9

Genome editing

Missense mutation

Nonsense

CRISPR

Mutation

Cancer research

Computational biology

Genetics

Genome

Nonsense-mediated decay

RNA splicing

Internal medicine

Medicine

Immunotherapy

Nonsense mutation

Disease

Cancer

Pathology

Nature Genetics

Premature termination codons (PTCs) can result in the production of truncated proteins or the degradation of messenger RNAs by nonsense-mediated mRNA decay (NMD). Which of these outcomes occurs can alter the effect of a mutation, with the engagement of NMD being dependent on a series of rules. Here, by applying these rules genome-wide to obtain a resource called NMDetective, we explore the impact of NMD on genetic disease and approaches to therapy. First, human genetic diseases differ in whether NMD typically aggravates or alleviates the effects of PTCs. Second, failure to trigger NMD is a cause of ineffective gene inactivation by CRISPR-Cas9 gene editing. Finally, NMD is a determinant of the efficacy of cancer immunotherapy, with only frameshifted transcripts that escape NMD predicting a response. These results demonstrate the importance of incorporating the rules of NMD into clinical decision-making. Moreover, they suggest that inhibiting NMD may be effective in enhancing cancer immunotherapy.

The impact of nonsense-mediated mRNA decay on genetic disease, gene editing and cancer immunotherapy