Quantitative real time polymerase chain reaction (q RT‐PCR ) and RNA scope in situ hybridization ( RNA ‐ ISH ) as effective tools to diagnose feline herpesvirus‐1‐associated dermatitis

Felid herpesvirus type 1 (FHV-1)-associated dermatitis is characterized by facial and nasal involvement; clinical and histopathological manifestations may overlap with other dermatitides.To evaluate the realibility of qRT-PCR-2-ΔΔCq and RNAscope in situ hybridization (RNA-ISH) methods to diagnose FHV-1-associated dermatitis, in formalin-fixed paraffin-embedded (FFPE) tissues.Sixteen FFPE samples from cats with facial dermatitis and four controls...