In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation

eLife7.70
Volume: 8
Published: Aug 9, 2019
Abstract
Previously, we reported the first oxygen partial pressure (Po2) measurements in the brain of awake mice, by performing two-photon phosphorescence lifetime microscopy at micrometer resolution (Lyons et al., 2016). However, this study disregarded that imaging through a cranial window lowers brain temperature, an effect capable of affecting cerebral blood flow, the properties of the oxygen sensors and thus Po2 measurements. Here, we show that in...
Paper Details
Title
In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation
Published Date
Aug 9, 2019
Journal
Volume
8
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