Objective To assess the efficacy of the human amniotic membrane ( hAM ) to treat ( HMMH ) associated with retinal detachment ( RD ). Material and Methods Ten eyes of 10 patients with recurrent HMMH and RD , who had already undergone one or more pars plana vitrectomy ( PPV ), underwent a PPV with an hAM plug implanted in the macular hole. The initial five patients enrolled were tamponaded with ( SO ) while the subsequent five patients with 10% octafluoropropane (C 3 F 8 ). Silicon oil was removed in all five patients 2 months later. No statistical differences were reported between the two groups. Results Final retinal reattachment was achieved in all the patients. BCVA improved from 1.73 log MAR to 0.94 log MAR after 6 months. No adverse events were registered during follow‐up. Conclusion An hAM plug is an efficient substrate to manage HMMH associated with RD resulting in encouraging visual acuity recovery.