Biotechnology

Virus

Nicotiana benthamiana

Biology

Immunology

Biochemistry

Chikungunya

In vitro

Alphavirus

Virology

Antibody

In vivo

Glycosylation

Monoclonal antibody

Microbiology

Plant Biotechnology Journal

Summary Chikungunya virus ( CHIKV ) is a mosquito‐transmitted alphavirus, and its infection can cause long‐term debilitating arthritis in humans. Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections. In this study, we explored the feasibility of using an anti‐ CHIKV monoclonal antibody ( mA b) produced in wild‐type ( WT ) and glycoengineered (∆ XFT ) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model. CHIKV mA b was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme. While mA b produced in ∆ XFT carried a single N‐glycan species at the Fc domain, namely GnGn structures, WT produced mA b exhibited a mixture of N‐glycans including the typical plant GnGn XF 3 glycans, accompanied by incompletely processed and oligomannosidic structures. Both WT and ∆ XFT plant‐produced mA bs demonstrated potent in vitro neutralization activity against CHIKV . Notably, both mA b glycoforms showed in vi...

<i>In vitro and in vivo</i> efficacy of anti‐chikungunya virus monoclonal antibodies produced in wild‐type and glycoengineered <i>Nicotiana benthamiana</i> plants

In vitro and in vivo efficacy of anti‐chikungunya virus monoclonal antibodies produced in wild‐type and glycoengineered Nicotiana benthamiana plants