Enzymatic reconstruction of glycosaminoglycan oligosaccharides

The transglycosylation mechanism of testicular hyaluronidase, which is an endo-β-N-acetylhexosaminidase, was investigated with the aim of performing enzymatic synthesis of glycosaminoglycan (GAG) sugar chains. It was found that disaccharide units (glucuronic acid β1-3-N-accetylglucosamine β1-4) are successively released from the nonreducing terminal of a donor hyaluronic acid (HA) and rapidly transferred to the glucuronic acid residue at the...