Non-covalent albumin-binding ligands for extending the circulating half-life of small biotherapeutics

Published on Jun 6, 2019in MedChemComm2.807
· DOI :10.1039/C9MD00018F
Alessandro Zorzi5
Estimated H-index: 5
(EPFL: École Polytechnique Fédérale de Lausanne),
Sara Linciano2
Estimated H-index: 2
(Ca' Foscari University of Venice),
Alessandro Angelini18
Estimated H-index: 18
(Ca' Foscari University of Venice)
Peptides and small protein scaffolds are gaining increasing interest as therapeutics. Similarly to full-length antibodies, they can bind a target with a high binding affinity and specificity while remaining small enough to diffuse into tissues. However, despite their numerous advantages, small biotherapeutics often suffer from a relatively short circulating half-life, thus requiring frequent applications that ultimately restrict their ease of use and user compliance. To overcome this limitation, a large variety of half-life extension strategies have been developed in the last decades. Linkage to ligands that non-covalently bind to albumin, the most abundant serum protein with a circulating half-life of ∼19 days in humans, represents one of the most successful approaches for the generation of long-lasting biotherapeutics with improved pharmacokinetic properties and superior efficacy in the clinic.
📖 Papers frequently viewed together
355 Citations
262 Citations
1,492 Citations
#1Cai Read (University of Cambridge)H-Index: 6
#2Peiran Yang (University of Cambridge)H-Index: 13
Last. Janet J. Maguire (University of Cambridge)H-Index: 47
view all 11 authors...
: The apelin receptor is a potential target in the treatment of heart failure and pulmonary arterial hypertension where levels of endogenous apelin peptides are reduced but significant receptor levels remain. Our aim was to characterise the pharmacology of a modified peptide agonist, MM202, designed to have high affinity for the apelin receptor and resistance to peptidase degradation and linked to an anti-serum albumin domain antibody (AlbudAb) to extend half-life in the blood. In competition, b...
6 CitationsSource
#1James M. Kelly (Cornell University)H-Index: 10
#2Alejandro Amor-Coarasa (Cornell University)H-Index: 10
Last. John W. Babich (Cornell University)H-Index: 63
view all 7 authors...
: Despite significant gains in the treatment of metastatic castration-resistant prostate cancer by radioligands targeting prostate-specific membrane antigen (PSMA), 30% of patients never respond to therapy. One possible explanation is insufficient dose delivery to the tumor because of suboptimal pharmacokinetics. We have recently described RPS-063, a trifunctional ligand targeting PSMA with high uptake in LNCaP xenograft tumors but also in kidneys. We aimed to use structural modifications to inc...
16 CitationsSource
#1David Stepensky (BGU: Ben-Gurion University of the Negev)H-Index: 22
: Toxins and venoms produced by different organisms contain peptides that have evolved to have highly selective and potent pharmacological effects on specific targets for protection and predation. Several toxin-derived peptides have become drugs and are used for the management of diabetes, hypertension, chronic pain, and other medical conditions. Despite the similarity in their composition (amino acids as the building blocks), toxin-derived peptide drugs have very profound differences in their s...
11 CitationsSource
#2Martina Benešová (ETH Zurich)H-Index: 17
Last. Cristina Müller (ETH Zurich)H-Index: 38
view all 6 authors...
Recently, we developed an albumin-binding radioligand (177Lu–PSMA–ALB-56), which showed higher PSMA-specific tumor uptake in mice than the previously developed 177Lu–PSMA-617 under the same experimental conditions. Such a radioligand may be of interest also for PET imaging, possibly enabling better visualization of even small metastases at late time-points after injection. The aim of this study was, therefore, to modify PSMA–ALB-56 by exchanging the DOTA chelator with a NODAGA chelator for stabl...
12 CitationsSource
#1Esben M. Bech (UCPH: University of Copenhagen)H-Index: 5
#2Søren L. PedersenH-Index: 15
Last. Knud J. Jensen (UCPH: University of Copenhagen)H-Index: 40
view all 3 authors...
Strategies for half-life extension are often required in the design of new biopharmaceuticals. This Viewpoint focuses on chemical moieties that convey protraction by albumin binding or by self-assembly to form larger structures, with GLP-1 and insulin as examples.
37 CitationsSource
#1Junxia Wei (NIH: National Institutes of Health)H-Index: 2
#1Junxia Wei (NIH: National Institutes of Health)H-Index: 2
Last. Ira Pastan (NIH: National Institutes of Health)H-Index: 190
view all 8 authors...
Recombinant immunotoxins (RITs) are chimeric proteins consisting of a Fv that binds to a cancer cell and a portion of a protein toxin. One of these, Moxetumomab pasudotox, was shown to be effective in treating patients with some leukemias, where the cells are readily accessible to the RIT. However, their short half-life limits their efficacy in solid tumors, because penetration into the tumors is slow. Albumin and agents bound to albumin have a long half-life in the circulation. To increase the ...
26 CitationsSource
#1James M. Kelly (Cornell University)H-Index: 10
#2Alejandro Amor-Coarasa (Cornell University)H-Index: 10
Last. John W. Babich (Cornell University)H-Index: 63
view all 9 authors...
Purpose Treatment of late-stage prostate cancer by targeted radiotherapeutics such as 131I-MIP-1095 and 177Lu-PSMA-617 has shown encouraging early results. Lu-177 is preferred to I-131 in clinical settings, but targeted radioligand therapy (RLT) with 177Lu-PSMA-617 has not reached its full potential due to insufficient dose delivery to the tumor. We recently developed a dual-targeting radioiodinated ligand, RPS-027, that targets PSMA and uses albumin binding to enable good tumor uptake and signi...
23 CitationsSource
#1Yizhou Li (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 13
#2Roberto De Luca (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 5
Last. Dario Neri (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 99
view all 7 authors...
In nature, specific antibodies can be generated as a result of an adaptive selection and expansion of lymphocytes with suitable protein binding properties. We attempted to mimic antibody–antigen recognition by displaying multiple chemical diversity elements on a defined macrocyclic scaffold. Encoding of the displayed combinations was achieved using distinctive DNA tags, resulting in a library size of 35,393,112. Specific binders could be isolated against a variety of proteins, including carbonic...
51 CitationsSource
#1Antoine Henninot (Ferring Pharmaceuticals)H-Index: 2
#2James C. Collins (Ferring Pharmaceuticals)H-Index: 1
Last. John M. Nuss (Ferring Pharmaceuticals)H-Index: 1
view all 3 authors...
Over the past decade, peptide drug discovery has experienced a revival of interest and scientific momentum, as the pharmaceutical industry has come to appreciate the role that peptide therapeutics can play in addressing unmet medical needs and how this class of compounds can be an excellent complement or even preferable alternative to small molecule and biological therapeutics. In this Perspective, we give a concise description of the recent progress in peptide drug discovery in a holistic manne...
332 CitationsSource
#1Conor McMahon (Harvard University)H-Index: 10
#2Alexander S. Baier (Harvard University)H-Index: 3
Last. Andrew C. Kruse (Harvard University)H-Index: 44
view all 12 authors...
Camelid single-domain antibody fragments (‘nanobodies’) provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin VHH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs). Nearly all nanobodies available to date have been obta...
147 CitationsSource
Cited By35
#1Carsten HöltkeH-Index: 16
#2Wael AlsibaiH-Index: 2
Last. Anne HelfenH-Index: 8
view all 0 authors...
The biodistribution of medical imaging probes depends on the chemical nature of the probe and the preferred metabolization and excretion routes. Especially targeted probes, which have to reach a certain protein structure, have to be guided to the tissue of interest. Small molecules have rather short half-lives while antibodies reside inside the organism for a longer period of time and an excretion via kidneys and bladder is faster than a hepatobiliary excretion. Therefore, small hydrophilic prob...
#1Ashan Wijesinghe (MUN: Memorial University of Newfoundland)
#2Sarika Kumari (MUN: Memorial University of Newfoundland)
Last. Valerie Booth (MUN: Memorial University of Newfoundland)H-Index: 16
view all 3 authors...
While peptides can be excellent therapeutics for several conditions, their limited null in vivo null half-lives have been a major bottleneck in the development of therapeutic peptides. Conjugating the peptide to an inert chemical moiety is a strategy that has repeatedly proven to be successful in extending the half-life of some therapeutics. This systematic review and meta-analysis was conducted to examine the available literature and assess it in an unbiased manner to determine which conjugates...
#1Benjamin J. Tombling (UQ: University of Queensland)H-Index: 3
#2Yuhui Zhang (UQ: University of Queensland)
Last. Conan K. Wang (UQ: University of Queensland)H-Index: 30
view all 5 authors...
Abstract null null Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a clinically validated target for treating cardiovascular disease (CVD) due to its involvement in cholesterol metabolism. Although approved monoclonal antibodies (alirocumab and evolocumab) that inhibit PCSK9 function are very effective in lowering cholesterol, their limitations, including high treatment costs, have so far prohibited widespread use. Accordingly, there is great interest in alternative drug modalities to a...
#1Aurélie Rondon (UCL: Université catholique de Louvain)H-Index: 1
#2Sohaib Mahri (UCL: Université catholique de Louvain)H-Index: 3
Last. Rita Vanbever (UCL: Université catholique de Louvain)H-Index: 34
view all 5 authors...
#1Benjamin J. Tombling (UQ: University of Queensland)H-Index: 3
#2Carmen Lammi (University of Milan)H-Index: 19
Last. Conan K. Wang (UQ: University of Queensland)H-Index: 30
view all 6 authors...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a clinically validated target for treating hypercholesterolemia. Peptide-based PCSK9 inhibitors have attracted pharmaceutical interest, but the effect of multivalency on bioactivity is poorly understood. Here we designed bivalent and tetravalent dendrimers, decorated with the PCSK9 inhibitory peptides Pep2-8[RRG] or P9-38, to study relationships between peptide binding affinity, peptide valency, and PCSK9 inhibition. Increased valency resu...
2 CitationsSource
#1Conor Mcquaid (OU: Open University)H-Index: 1
#2Andrea Halsey (OU: Open University)
Last. David Male (OU: Open University)H-Index: 36
view all 5 authors...
The ability to target therapeutic agents to specific tissues is an important element in the development of new disease treatments. The transferrin receptor (TfR) is one potential target for drug delivery, as it expressed on many dividing cells and on brain endothelium, the key cellular component of the blood-brain barrier. The aim of this study was to compare a set of new and previously-described polypeptides for their ability to bind to brain endothelium, and investigate their potential for tar...
#1Carsten HöltkeH-Index: 16
#2Martin GrewerH-Index: 1
Last. Anne HelfenH-Index: 8
view all 6 authors...
The biodistribution of molecular imaging probes or tracers mainly depends on the chemical nature of the probe and the preferred metabolization and excretion routes. Small molecules have rather short half-lives while antibodies reside inside the organism for a longer period of time. An excretion via kidneys and bladder is faster than a mainly hepatobiliary elimination. To manipulate the biodistribution behavior of probes, different strategies have been pursued, including utilizing serum albumin a...
1 CitationsSource
#1Soghra Bagheri (Kermanshah University of Medical Sciences)H-Index: 3
#2Ali Akbar Saboury (UT: University of Tehran)H-Index: 54
A common problem in many cancers is the resistance of some patients to common drugs or relapse. Hypoalbuminemia has been reported in some of resistant cancer patients.This article evaluates the usefulness of albumin in the treatment of drug-resistant cancers with hypoalbuminemia based on available evidences.Rapid metabolism and drug excretion from the body is one of the causes of drug resistance. Albumin is the major plasma protein to which almost all drugs are bound. There is some evidence that...
#1Dmitry A. Gruzdev (UrFU: Ural Federal University)H-Index: 2
#2Galina L. LevitH-Index: 15
Last. Valery N. Charushin (UrFU: Ural Federal University)H-Index: 29
view all 4 authors...
Abstract Derivatives of dicarba-closo-dodecaboranes and dicarba-nido-undecaboranes (carboranes) are of increasing interest for modern medicinal chemistry and materials science. The combination of carborane residues and amino acid fragments in one structure can serve as a basis for obtaining promising medicinal agents (primarily, for boron neutron capture therapy), as well as for design the materials with unique properties. For the first time, this review summarizes and analyzes information on th...
1 CitationsSource