Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent

Deep eutectic solvent (DES), has been considered as a new type of green solvent applied in enzymatic systems. Here, we reported DES-buffer co-solvent as a novel reaction medium for high efficient synthesis of cefaclor by penicilin acylase immobilized on magnetic nanocrystalline cellulose. Effect of DES composition, DES-buffer ratio, temperature, pH, substrate ratio and substrate concentration was systematically investigated. In co-solvent...