Biology

Cell culture

Lipopolysaccharide

Diterpene

Biological activity

Nitric oxide

Biochemistry

Botany

Chemical structure

Stereochemistry

Cytotoxicity

Immunology

Genetics

Organic chemistry

Chemical synthesis

Pharmacognosy

Euphorbiaceae

In vitro

Cytotoxic T cell

Pharmacology

IC50

Medicine

Chemistry

Traditional medicine

Journal of Natural Products

Seven new Securinega alkaloids, securingines A–G (1–7), together with seven known analogues (8–14), were isolated from the twigs of Securinega suffruticosa. Their chemical structures were elucidated by a combined approach of spectroscopic analysis, chemical methods, ECD calculations, and DP4+ probability analysis. The full NMR assignments and the absolute configuration of compound 8 are also reported. In addition, all the isolated phytochemicals (1–14) were assessed for their cytotoxic, anti-inflammatory, and potential neuroprotective activities. Compound 4 showed cytotoxic activity (IC50 values of 1.5–6.8 μM) against four human cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15). Compounds 3, 10, 12, and 13 showed potent inhibitory effects on nitric oxide production (IC50 values of 12.6, 12.1, 1.1, and 7.7 μM, respectively) in lipopolysaccharide-stimulated murine microglia BV-2 cells. Compound 5 exhibited a nerve growth factor production effect (172.6 ± 1.2%) in C6 glioma cells at 20 μg/mL.

<i>Securinega</i> Alkaloids from the Twigs of <i>Securinega suffruticosa</i> and Their Biological Activities

Securinega Alkaloids from the Twigs of Securinega suffruticosa and Their Biological Activities