Thermostability enhancement of the α-carbonic anhydrase fromSulfurihydrogenibium yellowstonenseby using the anchoring-and-self-labelling-protein-tagsystem (ASL^tag)

Carbonic anhydrases (CAs, EC 4.2.1.1) are a superfamily of ubiquitous metalloenzymes present in all living organisms on the planet. They are classified into seven genetically distinct families and catalyse the hydration reaction of carbon dioxide to bicarbonate and protons, as well as the opposite reaction. CAs were proposed to be used for biotechnological applications, such as the post-combustion carbon capture processes. In this context, there...