Gram

Gene

Quantum mechanics

Biotechnology

Bacteria

Biology

Gram-negative bacteria

Biochemistry

Gram staining

Genetics

In vivo

Physics

Optics

Microbiology

Fluorescence

Escherichia coli

Chemistry

Gram-positive bacteria

Angewandte Chemie International Edition

The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram-positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application.

Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria