Engineering

Ecology

Biology

Chromatography

Steam explosion

Cellulose

Biochemistry

Hemicellulose

Botany

Food science

Substrate (aquarium)

Extraction (chemistry)

Organic chemistry

Enzyme

Chemistry

Phosphoric acid

Xylose

Bamboo

Pulp and paper industry

Fermentation

Lignin

Bioresource Technology

In this work, a mild and facile post-extraction using different reagents was evaluated to overcome these recalcitrance for improving the enzymatic digestibility of acid-pretreated bamboo residues by removing the lignin and disrupting its inhibitory properties. Results showed that the enzymatic digestibility of acid-pretreated bamboo residues can be improved from 15.4% to 61.4%, 59.7%, and 42.8% by room temperature post-extraction with phosphoric acid, urea, and ethanol, respectively. Several compelling correlations (R2 > 0.5) were observable between enzymatic digestibility and structural changes, including delignification, reducing of substrate hydrophobicity, altering cellulose crystallinity, and elevations to the residual lignin syringyl-to-guaiacyl (S/G) ratio and functional groups. The results serve as a demonstration of the downstream value that can be gained when coupling a post-extraction process with acid pretreatment of bamboo residues, resulting in greater fermentable sugar production.

Coupling the post-extraction process to remove residual lignin and alter the recalcitrant structures for improving the enzymatic digestibility of acid-pretreated bamboo residues