Protein denaturation at the air-water interface and how to prevent it

eLife6.40
Volume: 8
Published: Apr 1, 2019
Abstract
Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in unsupported films of aqueous solution, which can result in partial or complete denaturation. We investigated the structure of yeast fatty acid synthase at the air-water interface by electron cryo-tomography and single-particle image processing. Around 90% of complexes adsorbed to the...
Paper Details
Title
Protein denaturation at the air-water interface and how to prevent it
Published Date
Apr 1, 2019
Journal
Volume
8
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