Pathology

Epoxy

Organic chemistry

Triclocarban

Bisphenol A

Triclosan

Biology

Environmental chemistry

Predation

Chemistry

Ecology

Seabird

Paraben

Nonylphenol

Microplastics

Endocrine disruptor

Phthalate

Preservative

Food science

Endocrine system

Biochemistry

Hormone

Medicine

Environmental Science & Technology

Plastic is ingested by over 100 bird species and 40 fish species. Once ingested, plastic may release endocrine-disrupting plastic additives in the animal; however, amounts transferred are poorly characterized. We exposed 16 commonly ingested plastic items to fish and seabird laboratory gut mimic models using the digestive enzyme pepsin at pH 2 and shook them for 16 h at either 28 °C (in saltwater) for fish or 40 °C (in freshwater) for seabirds. Gut liquid was then evaluated for estrogen receptor activity using an in vitro cell line, and plastic-additive concentrations were quantified using ultrahigh-performance liquid chromatography/tandem mass spectrometry. Both seabird (p < 0.0001) and fish gut conditions (p < 0.0001) significantly enhanced the biological estrogenicity of expanded polystyrene, polyethylene shopping bag, and polypropylene string relative to controls, resulting in up to a 10.6-fold increase in estrogenicity. Out of 12 plastic additives analyzed, bisphenol A (BPA) (204 ± 129%) and diethylhexyl phthalate (DEHP) (175 ± 97%) concentrations were significantly increased in seabird gut conditions relative to control and butylbenzyl phthalate (BBP) (132 ± 68%) was significantly increased in fish gut conditions relative to control. BPA, DEHP, and BBP did not adequately account for the increase in biological estrogenicity, suggesting that uncharacterized plastic additives may have been enhanced by gut conditions.

Fish and Seabird Gut Conditions Enhance Desorption of Estrogenic Chemicals from Commonly-Ingested Plastic Items