Pathology

High-performance liquid chromatography

Extraction (chemistry)

Chemistry

Traditional Chinese medicine

Alternative medicine

Scutellaria baicalensis

Active ingredient

Ingredient

Food science

Chromatography

Medicine

Pharmacology

Journal of Applied Biological Chemistry

Scutellaria baicalensis Georgi (Scutellariae Radix) has been widely used as a dietary ingredient and traditional herbal medicine such as diuretic, hyperlipidemia, antibacterial, antiallergy, anti-inflammatory and anticancer properties. In this study, the isolation of biomarkers or bioactive compounds from complex S. baicalensis extracts represents an essential step for de novo identification and bioactivity assessment. The bioactive fraction consisted of eight compounds which was chromatographed on an analytical high performance liquid chromatography column using two different gradient runs. A simulative replacement of the analytical column with a medium pressure liquid chromatography and open column allowed the determination of gradient profile to allow sufficient separation in the preparative scale. From the optimized method, eight standard compounds have been identified in the fractions. In addition, MS, UV, HRMS detection was provided by ultraperformance liquid chromatographyequadrupole time-of-flight mass spectrometry (UPLC-QTof-MS) of all fractions. Therefore, this scale up procedure was successfully applied to a S. baicalensis extract.

Rational and efficient approach to the preparation of the active fractions of <i>Scutellaria baicalensis</i>

Rational and efficient approach to the preparation of the active fractions of Scutellaria baicalensis