Diminished apoptosis in hypoxic porcine retina explant cultures through hypothermia

Simulation of hypoxic processes in vitro can be achieved through cobalt chloride (CoCl 2 ), which induces strong neurodegeneration. Hypoxia plays an important role in the progression of several retinal diseases. Thus, we investigated whether hypoxia can be reduced by hypothermia. Porcine retinal explants were cultivated for four and eight days and hypoxia was mimicked by adding 300 µM CoCl 2 from day one to day three. Hypothermia treatment (30...