Gene

Akkermansia muciniphila

Biology

Biochemistry

Mushroom

Immunoglobulin A

Food science

Polysaccharide

Bacteroidetes

Firmicutes

16S ribosomal RNA

Immunology

Microbiome

Bifidobacterium

Bioinformatics

Immune system

Immunoglobulin G

Lactobacillus

Prebiotic

Microbiology

Akkermansia

Flammulina

Gut flora

Chemistry

Fermentation

Journal of Functional Foods

A crude polysaccharide derived from Flammulina velutipes (FVP), mainly consisting of glucose linked with β-glycosidic bonds, was administered to 6 week-old male ICR mice by gavage for five weeks, to investigate the effects on mice intestinal microbiota and immune response. The pH of the intestinal compartments decreased while short chain fatty acids concentrations increased in FVP-fed groups, in a dose dependent manner. High throughput sequencing revealed the enrichment in the diversity and an alteration in the composition of the fecal microbiota in the FVP fed mice. A significant decrease in the Firmicutes/Bacteroidetes ratio, relative abundances of Lactobacillus and Akkermansia were related to the energy absorption. The relative abundances of Porphyromonadaceae and Bacteroidaceae positively correlated with an increase in serum immunoglobulin and cytokines. This study indicated that this crude polysaccharide from F. velutipes could modulate the gut microbiota and represented a potential capacity of obesity suppression and immune regulation.

Effects of Flammulina velutipes polysaccharide on immune response and intestinal microbiota in mice