Proximal marine strata of the North American Western Interior Basin (WIB) preserve a rich record of biotic turnover during Oceanic Anoxic Event 2 (OAE2; ca. 94 Ma), a pronounced Late Cretaceous carbon cycle perturbation interpreted to reflect global warming, widespread hypoxia, and possible ocean acidification. To develop a more robust synthesis of paleobiologic and geochemical data sets spanning this Earth-life transition, we drilled the 131 m Smoky Hollow #1 Core (SH#1), on the Kaiparowits Plateau of southern Utah, USA, recovering the Cenomanian–Turonian Boundary (CTB) interval in the Tropic Shale Formation. A 17.5 m positive excursion in high-resolution bulk carbon isotope chemostratigraphy (δ13Corg) of SH#1 characterizes the most expanded OAE2 record recovered from the mid-latitudes of the WIB. Depleted values in a paired carbonate carbon isotope (δ13Ccarb) chemostratigraphy cyclically punctuate the OAE2 excursion. These depletions correspond to intervals in the core with a higher degree of carbonate diagenesis and correlate well to an existing sequence stratigraphic framework of flooding surfaces in the shoreface facies of the Markagunt Plateau (∼100 km west). We detect statistically significant evidence for astronomical cycles in the δ13Ccarb data set, imparted by diagenesis at flooding surfaces, and develop a floating astronomical time scale (ATS) for the study interval. Stable eccentricity cycles (405 k.y.) align with stratigraphic sequences and associated trends in sedimentation rate, and short eccentricity cycles (∼100 k.y.) pace nested parasequences. These results confirm an astronomical signal and, therefore, climatic forcing of relative sea level during OAE2 in the WIB. Furthermore, cross-basin correlation of the ATS and expanded δ13C chemostratigraphy of SH#1 suggests that these transgressive-regressive parasequences modulated siliciclastic sediment delivery in the seaway and contributed to deposition of prominent rhythmically bedded CTB units across the WIB, including the Bridge Creek Limestone. Th...