The utilization of bio-mordants to make natural coloring process more eco-friendly is gaining worldwide fame, whereas the addition of microwave treatment has added value to the extraction of colorant under mild conditions. The Current study has been undertaken to isolate tannin from neem (Azadirachta indica) bark under the impact of microwave radiation for the dyeing of mordanted (chemical and bio) silk fabric. For this purpose, the extraction of colorant (tannin) has been carried out in various media and dyeing variables have been optimized. For making the process more sustainable and clean, extracts ofbio-mordants such as turmeric (Curcuma longa), henna (Lawsonia inermis), pomegranate (Punica granatum) and acacia (Acacia nilotica) have been employed at given conditions. It is found that microwave radiation for 2 min. to acidified methanolic extract of 2 pH obtained from 6 g powder has given good color strength in irradiated silk when it is used to dye at 75°C for 65 min. by employing 3 g/100 mL of Glauber’s salt the as exhausting agent. It is concluded that microwave treatment has not only enhanced the extraction of tannin from neem bark but also improved the color characteristics under low conditions.