Mutant

Gene expression

Biology

Promoter

Gene

Transcription (linguistics)

Abscisic acid

Cell biology

Chromatin immunoprecipitation

Philosophy

Genetics

Chromatin

Transcription factor

Linguistics

Arabidopsis

PLANT PHYSIOLOGY

Arabidopsis (Arabidopsis thaliana) GARP (Golden2, ARR-B, Psr1) family transcription factors, GOLDEN2-LIKE1 and -2 (GLK1/2), function in different biological processes; however, whether and how these transcription factors modulate the response to abscisic acid (ABA) remain unknown. In this study, we used a glk1 glk2 double mutant to examine the role of GLK1/2 in the ABA response. The glk1 glk2 double mutant displayed ABA-hypersensitive phenotypes during seed germination and seedling development and an osmotic stress-resistant phenotype during seedling development. Genome-wide RNA sequencing analysis of the glk1 glk2 double mutant revealed that GLK1/2 regulate several ABA-responsive genes, including WRKY40, in the presence of ABA. Chromatin immunoprecipitation and gel retardation assays showed that GLK1/2 directly associate with the WRKY40 promoter via the recognition of a consensus sequence. Additionally, RNA sequencing analysis of the glk1 glk2 double mutant and wrky40 single mutant revealed that GLK1/2 and W...

GOLDEN2-LIKE Transcription Factors Regulate <i>WRKY40</i> Expression in Response to Abscisic Acid

GOLDEN2-LIKE Transcription Factors Regulate WRKY40 Expression in Response to Abscisic Acid