A compilation of the new developments in terms of detection, detections systems and detection strategies in Ultraviolet-Visible (UV-Vis) spectrophotometry is presented and discussed. It is shown that this evolution has promoted the use of UV-Vis spectrophotometry as a simple, sensitive, reliable, and low cost technique, that allows the determination of very low concentrations of compounds, and the use of very small amounts of samples. The versatility, and the portability of the developed equipment and strategies, have also contributed for the continuous use of UV-Vis spectrophotometry , over the years. By the examples of its use in diverse fields such as agriculture, food, clinical, pharmaceutical, and environmental is highly expectable that detection in UV-Vis spectrophotometry, continues to evolve and to promote this kind of applications.