Gene

Cell biology

Reproductive technology

In vitro fertilisation

Biology

Human fertilization

Zygote

Genome editing

Offspring

Endocrinology

Maternal to zygotic transition

Genetics

In vivo

Embryo

Genome

Embryonic stem cell

Embryogenesis

Oviduct

Andrology

Medicine

Reprogramming

Pregnancy

Nature Communications

Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi)genome in preparation for the reacquisition of (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi)genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and pre-implantation development, which could improve the quality and (epi)genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development.

An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming