Cell biology

Chemical physics

Biofilm

Bacteria

Biology

Vibrio cholerae

Materials science

Dynamics (music)

Acoustics

Active matter

Genetics

Physics

Nanotechnology

Biological system

Biophysics

Nature Physics

Surface-attached bacterial biofilms are self-replicating active liquid crystals and the dominant form of bacterial life on Earth1–4. In conventional liquid crystals and solid-state materials, the interaction potentials between the molecules that comprise the system determine the material properties. However, for growth-active biofilms it is unclear whether potential-based descriptions can account for the experimentally observed morphologies, and which potentials would be relevant. Here, we have overcome previous limitations of single-cell imaging techniques5,6 to reconstruct and track all individual cells inside growing three-dimensional biofilms with up to 10,000 individuals. Based on these data, we identify, constrain and provide a microscopic basis for an effective cell–cell interaction potential, which captures and predicts the growth dynamics, emergent architecture and local liquid-crystalline order of Vibrio cholerae biofilms. Furthermore, we show how external fluid flows control the microscopic structure and three-dimensional morphology of biofilms. Our analysis implies that local cellular order and global biofilm architecture in these active bacterial communities can arise from mechanical cell–cell interactions, which cells can modulate by regulating the production of particular matrix components. These results establish an experimentally validated foundation for improved continuum theories of active matter and thereby contribute to solving the important problem of controlling biofilm growth. Single-cell tracking of up to 10,000 bacteria reveals the structure and dynamics of 3D biofilms—providing evidence to suggest that both local ordering and global biofilm architecture emerge from mechanical interactions.

Emergence of three-dimensional order and structure in growing biofilms