Review of recent developments in GC-MS approaches to metabolomics-based research.

Published on Nov 17, 2018in Metabolomics2.881
· DOI :10.1007/S11306-018-1449-2
David J. Beale25
Estimated H-index: 25
(CSIRO: Commonwealth Scientific and Industrial Research Organisation),
Farhana R. Pinu16
Estimated H-index: 16
(Plant & Food Research)
+ 7 AuthorsDaniel A. Dias24
Estimated H-index: 24
(RMIT: RMIT University)
Background Metabolomics aims to identify the changes in endogenous metabolites of biological systems in response to intrinsic and extrinsic factors. This is accomplished through untargeted, semi-targeted and targeted based approaches. Untargeted and semi-targeted methods are typically applied in hypothesis-generating investigations (aimed at measuring as many metabolites as possible), while targeted approaches analyze a relatively smaller subset of biochemically important and relevant metabolites. Regardless of approach, it is well recognized amongst the metabolomics community that gas chromatography-mass spectrometry (GC–MS) is one of the most efficient, reproducible and well used analytical platforms for metabolomics research. This is due to the robust, reproducible and selective nature of the technique, as well as the large number of well-established libraries of both commercial and ‘in house’ metabolite databases available.
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