Simultaneous determination of bioactive flavonoids of Hoveniae Semen in rat plasma by LC-MS/MS: Application to a comparative pharmacokinetic study.

Published on Jan 1, 2019in Journal of Chromatography B3.004
· DOI :10.1016/J.JCHROMB.2018.11.012
Xueyan Yang2
Estimated H-index: 2
(SPU: Shenyang Pharmaceutical University),
Liye Yan1
Estimated H-index: 1
(SPU: Shenyang Pharmaceutical University)
+ 4 AuthorsZhiguo Yu16
Estimated H-index: 16
(SPU: Shenyang Pharmaceutical University)
Abstract A selective, sensitive and fully validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the determination of dihydromyricetin, dihydroquercetin, myricetin and quercetin in rat plasma after intragastric administration of Hoveniae Semen total flavonoids. Baicalin was selected as the internal standard. Analytes were extracted from the rat plasma by protein precipitation with acetonitrile and separated on a C18 chromatographic column (Agilent ZORBAX Eclipse Plus, 4.6 mm × 100 mm, 3.5 μm) using the mobile phase containing acetonitrile (A) and 0.1% formic acid-water (B) by gradient elution at 0.5 mL/min flow rate. A tandem mass spectrometer equipped with an electrospray ionization (ESI) source was used to detect analytes. The analytes were measured by multiple reaction monitoring (MRM) in the negative ionization mode. The lower limit of quantification of dihydromyricetin, dihydroquercetin, myricetin and quercetin were 0.70, 8.16, 1.62 and 0.56 ng/mL, respectively. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and the compounds were stable in rat plasma under all tested conditions. The approach was successfully applied to study pharmacokinetic characteristics of the four bioactive flavonoids in plasma after administering Hoveniae Semen total flavonoids intragastrically to rat. Further investigation was carried out to assess pharmacokinetic comparability of the four bioactive flavonoids after intragastric administration of Hoveniae Semen total flavonoids to mixture of flavonoids.
📖 Papers frequently viewed together
41 Citations
5 Citations
19 Citations
#1Yaoting Sun (Dalian Medical University)H-Index: 4
Recently, a new target Ca2+-binding protein SORCIN was reported to participate in multidrug resistance (MDR) in cancer. Here we aim to investigate whether dihydromyricetin (DMY), a dihydroflavonol compound with anti-inflamatory, anti-oxidant, anti-bacterial and anti-tumor actions, reverses MDR in MCF-7/ADR and K562/ADR and to elucidate its potential molecular mechanism. DMY enhanced cytotoxicity of adriamycin (ADR) by downregulating MDR1 mRNA and P-gp expression through MAPK/ERK pathway and also...
27 CitationsSource
#1Elias Atala (University of Chile)H-Index: 9
#2Jocelyn Fuentes (University of Chile)H-Index: 7
Last. Hernán Speisky (University of Chile)H-Index: 42
view all 4 authors...
Abstract Oxidation of a phenolic group in quercetin is assumed to compromise its antioxidant properties. To address this assumption, the ROS-scavenging, Folin-Ciocalteau- and Fe-reducing capacities of quercetin and thirteen structurally related flavonoids were assessed and compared with those of mixtures of metabolites resulting from their chemical and enzymatic oxidation. Regardless of the oxidation mode, the metabolites mixtures largely conserved the antioxidant properties of the parent molecu...
44 CitationsSource
#1Hoejin Kim (Ewha Womans University)H-Index: 1
#2You Jin Kim (Ewha Womans University)H-Index: 10
Last. Oran Kwon (Ewha Womans University)H-Index: 21
view all 7 authors...
Abstract Ethnopharmacological relevance Hovenia dulcis , known as the oriental raisin tree, is mainly found in East Asia. It has long been used as traditional folk remedies for alcohol intoxication. Aim of the study To examine the anti-hangover effect of Hovenia dulcis Thunb. fruit extract (HDE) in a randomized controlled crossover trial. Materials and methods Twenty-six eligible male adults with heterozygous ALDH2 (23.7±0.3 years old) consumed 360 mL of Korean Soju (50 g alcohol) together with ...
18 CitationsSource
#1Ra-Yeong Choi (SNU: Sunchon National University)H-Index: 8
#2Moon-Jae WooH-Index: 1
Last. Mi-Kyung Lee (SNU: Sunchon National University)H-Index: 45
view all 4 authors...
Abstract The anti-steatotic and anti-inflammatory effects of fruit water extract (FW) and seed ethanol extract (SE) of Hovenia dulcis Thunb. in chronic alcohol-fed rats were investigated. Rats were fed a liquid diet containing 36% calories from alcohol and orally administered FW or SE (300 and 500 mg/kg/day). Both FW and SE reduced hepatic lipid contents and droplets, serum lipid concentration and inflammatory markers (hs-CRP, TNF-α and IL-6) levels compared with the alcohol control group. Alcoh...
13 CitationsSource
#1Nagaraja Haleagrahara (JCU: James Cook University)H-Index: 20
#2Socorro Miranda-Hernandez (JCU: James Cook University)H-Index: 6
Last. Natkunam Ketheesan (JCU: James Cook University)H-Index: 27
view all 6 authors...
Abstract Quercetin, a bioactive flavonoid with anti-inflammatory, immunosuppressive, and protective properties, is a potential agent for the treatment of rheumatoid arthritis (RA). Collagen-induced arthritis (CIA) is the most commonly used animal model for studying the pathogenesis of RA. This study analysed the therapeutic role of quercetin in collagen-induced arthritis in C57BL/6 mice. The animals were allocated into five groups that were subjected to the following treatments: negative (untrea...
61 CitationsSource
#1Yingqi Xu (UM: University of Macau)H-Index: 8
#2Shengpeng Wang (UM: University of Macau)H-Index: 26
Last. Meiwan Chen (UM: University of Macau)H-Index: 51
view all 7 authors...
Dihydromyricetin Induces Apoptosis and Reverses Drug Resistance in Ovarian Cancer Cells by p53-mediated Downregulation of Survivin
25 CitationsSource
#1Shang Jyh Kao (Memorial Hospital of South Bend)H-Index: 1
#2Wei Jiunn Lee (TMU: Taipei Medical University)H-Index: 20
Last. Ming Hsien Chien (TMU: Taipei Medical University)H-Index: 29
view all 7 authors...
Nonsmall cell lung cancer (NSCLC) is the most common type of lung cancer with a high mortality rate and still remains a therapeutic challenge. A strategy for targeting NSCLC is to identify agents that are effective against NSCLC cells while sparing normal cells. Dihydromyricetin (DHM) is the major flavonoid component derived from Ampelopsis grossedentata, which has a long history of use in medicine. Herein, the molecular mechanisms by which DHM exerts its anticancer effects against NSCLC cells w...
32 CitationsSource
#1Yonghong Meng (SNNU: Shaanxi Normal University)H-Index: 9
#2Anping Su (SNNU: Shaanxi Normal University)H-Index: 3
Last. Yurong Guo (SNNU: Shaanxi Normal University)H-Index: 1
view all 8 authors...
This study was designed to investigate the inhibition effect and mechanism of total flavonoids, myricetin and quercetin extracted from Hovenia dulcis Thunb. on α-amylase and α-glucosidase in order to explore the potential use of Hovenia flavonoids in alleviating postprandial hyperglycemia. The results demonstrate that total flavonoids, myricetin, and quercetin were effective inhibitors of α-amylase with IC50 values of 32.8, 662 and 770 μg ml−1, respectively. And all three were effective inhibito...
68 CitationsSource
#7Seyed Mohammad Nabavi (BMSU: Baqiyatallah University of Medical Sciences)H-Index: 65
Abstract Liver diseases caused by viral hepatitis infections have a negative impact on global health. Approximately 30 million people in the USA and 29 million people in the European Union suffer from chronic liver disease. There are many kinds of diseases of the liver, caused by viruses, such as hepatitis A, hepatitis B and hepatitis C, or by certain drugs and poisons including excessive alcohol consumption. Many herbal medicines are used in traditional medicine for their protective and therape...
75 CitationsSource
Members of the family Picornaviridae, in particular, enteroviruses, represent a serious threat to human health. They are responsible for numerous pathologies ranging from mild disease to fatal outcome. Due to the limited number of safe and effective antivirals against enteroviruses, there is a need for search and development of novel drugs with various mechanisms of activity against enteroviruses-induced pathologies. We studied the effect of dihydroquercetin (DHQ), a flavonoid from larch wood, o...
18 CitationsSource
Cited By5
#1Gianluca Sferrazza (National Research Council)H-Index: 5
#2Gloria Brusotti (UNIPV: University of Pavia)H-Index: 14
Last. Pasquale Pierimarchi (National Research Council)H-Index: 21
view all 9 authors...
Hovenia dulcis Thunberg is an herbal plant, belonging to the Rhamnaceae family, widespread in west Asia, USA, Australia and New Zealand, but still almost unknown in Western countries. H. dulcis has been described to possess several pharmacological properties, such as antidiabetic, anticancer, antioxidant, anti-inflammatory and hepatoprotective, especially in the hangover treatment, validating its use as an herbal remedy in the Chinese Traditional Medicine. These biological properties are related...
1 CitationsSource
#1Wan Xu (Beijing University of Chinese Medicine)
#2Shaohong Chen (Beijing University of Chinese Medicine)H-Index: 1
Last. Yanmin Lv (Beijing University of Chinese Medicine)
view all 9 authors...
Abstract Objective The Flower of Lobed Kudzuvine [Pueraria lobata(Willd.)Ohwi; Gehua in Chinese; GH] and Japanese Raisin Tree Seed (Hovenia dulcis Thunnb.; Zhijuzi in Chinese; ZJZ) are herbs that have been used in China for the treatment of alcohol intoxication and liver diseases. We aimed to evaluate the hepatoprotective potential of a combination of these in mice with acute alcohol-induced liver injury, and to elucidate the mechanisms involved. Methods Male ICR mice were randomly allocated to ...
#1Dongyan Guo (Shaanxi University of Chinese Medicine)H-Index: 5
#2Juanli Dang (Shaanxi University of Chinese Medicine)H-Index: 1
Last. Junbo Zou (Shaanxi University of Chinese Medicine)H-Index: 4
view all 8 authors...
Abstract A rapid, sensitive, selective, and accurate UPLC–MS/MS method was developed and fully validated for the simultaneous determination of quercitrin, phloridzin, quercetin, and phloretin in rat plasma after oral administration of Malus hupehensis (Pamp.) Rehd extracts. The pharmacokinetic parameters of oral phloridzin monomer and phloridzin in the extract were also compared. Plasma samples were processed with a simple protein precipitation technique using methanol, followed by chromatograph...
4 CitationsSource
#1A. Vijaya Bhaskar Reddy (UTP: Universiti Teknologi Petronas)H-Index: 10
#2Muhammad Moniruzzaman (UTP: Universiti Teknologi Petronas)H-Index: 29
Last. Jafariah Jaafar (UTM: Universiti Teknologi Malaysia)H-Index: 12
view all 4 authors...
Abstract The importance of medicinal products originated from the plant materials has greatly increased in recent years. Among the different bioactive natural products, flavonoids are crucial compounds and are represented by over 8000 varieties. These flavonoid substances are found largely in the foods of plant sources, and they are subdivided into 13 categories including anthocyanidins, catechins, flavonols and flavones. Considering the promising protective effects of flavonoids against cardiov...
2 CitationsSource
#1Dan Liu (GDUT: Guangdong University of Technology)H-Index: 2
#2Yiqin Mao (GDUT: Guangdong University of Technology)H-Index: 3
Last. Xin-An Zeng (SCUT: South China University of Technology)H-Index: 41
view all 4 authors...
Abstract Background Dihydromyricetin (DMY) is an important plant flavonoid, which has received great attention due to its health-benefiting activities, including antioxidant, antimicrobial, anti-inflammatory, anticancer, antidiabetic and neuroprotective activities. DMY capsules have been sold in US as a nutraceutical supplement to prevent alcoholic hangovers. The major disadvantage associated with DMY is its chemical instability and poor bioavailability caused by the combined effects of its low ...
22 CitationsSource