Gamma‐H2AX immunofluorescence for the detection of tissue‐specific genotoxicity in vivo

The phosphorylation of histone H2AX in Serine 139 (gamma‐H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma‐H2AX is used widely as DNA damage marker in vitro , but its use for genotoxicity assessment in vivo has not been extensively investigated. Here, we developed an image analysis system for the precise quantification of the gamma‐H2AX signal, which we used...