Biology

RNA-binding protein

Gene

N6-Methyladenosine

RNA splicing

Cell biology

Molecular biology

Biochemistry

X chromosome

Methylation

Polyadenylation

Methyltransferase

Chemistry

Protein biosynthesis

Messenger RNA

XIST

Translation (biology)

Post-transcriptional regulation

X-inactivation

Journal of the American Chemical Society

Post-transcriptional gene expression regulation of RNA has emerged as a key factor that controls mammalian protein production. RNA trafficking, translation efficiency, and stability are all controlled at the transcript level. For example, in addition to the commonly known processing steps of capping, splicing, and polyadenylation, RNA can be chemically modified. In eukaryotes, N6-methyladenosine (m6A) is the most prevalent mRNA modification. While the writers, erasers, and readers for m6A are rapidly being uncovered and studied at the whole-cell level, their competitive interplay to regulate methylated RNA transcripts has yet to be elucidated. To address this limitation, we report the development of programmable dPspCas13b-m6A reader proteins to investigate the regulatory effects of specific readers on single transcripts in live cells. We fused the two most well-characterized m6A reader proteins, YTHDF1 and YTHDF2, to a catalytically inactive PspCas13b protein, which can target the reader to a specific RNA of...

Targeted m<sup>6</sup>A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs

Targeted m6A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs

Targeted m⁶A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs